Why in the News?
Scientists at the National Institute of Plant Genome Research (NIPGR), Delhi, have successfully used CRISPR-Cas9 gene editing technology to enhance phosphate uptake and utilization in japonica rice.
Back2Basics: CRISPR-Cas9 Gene Editing
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About Japonica Rice:
- Overview: Japonica is one of the two major cultivated rice subspecies, the other being Indica.
- Research Use: The Nipponbare variety of Japonica was used in recent gene-editing experiments.
- Why Japonica is Preferred in Studies:
- High regeneration potential in tissue culture
- Easier genetic transformation and faster growth in lab conditions
- Relevance to India: While not widely cultivated in India, Japonica acts as a model variety for testing before applying results to Indian Indica varieties.
Key Features of the Japonica Rice Study:
- Gene Editing Technique: Used CRISPR-Cas9 to edit a 30 base-pair repressor binding site on the promoter of the OsPHO1;2 gene.
- Outcomes of the Edit:
- Enhanced phosphate uptake from the soil
- Improved phosphate transport from root to shoot
- Yield increased by up to 40% using only 10% of the usual phosphate fertilizer
- Normal seed traits retained: size, shape, starch, and phosphate levels
- Significance: Demonstrated precise, minimal gene editing as a proof-of-concept that can be adapted to Indian rice varieties.
| [UPSC 2018] With reference to the Genetically Modified mustard (GM mustard) developed in India, consider the following statements:
1. GM mustard has the genes of a soil bacterium that give the plant the property of pest-resistance to a wide variety of pests. 2. GM mustard has the genes that allow the plant cross-pollination and hybridization. 3. GM mustard has been developed jointly by the IARI and Punjab Agricultural University. Which of the statements given above is/are correct? Options: (a) 1 and 3 only (b) 2 only * (c) 2 and 3 only (d) 1, 2 and 3 |
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