Why in the news?
Researchers from Rockefeller University introduced MagIC, a new method that allows cryo-EM to work with samples up to 100 times more dilute, making it easier to study rare or hard-to-purify molecules.
About Cryo-Electron Microscopy (Cryo-EM):
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What is MagIC (Magnetic Isolation and Concentration cryo-EM)?
- Overview: It is a new method developed by scientists in the U.S. to make it easier to study rare biological molecules under a special microscope called cryo-EM.
- Sampling involved: Normally, cryo-EM needs the molecules in a sample to be very concentrated, which is hard when the molecules are rare or hard to collect.
- MagIC solves this problem by using:
- Tiny magnetic beads (50 nanometers wide) that stick to the molecules researchers want to study.
- A magnet that pulls these beads together into one area.
- This way, even when the solution has less than 0.0005 milligrams per milliliter of the molecules, scientists can still get useful images.
Key Features of MagIC:
- Magnetic Pulling: After molecules stick to the tiny magnetic beads, a magnet pulls them into clusters, making them easier to see.
- Low Sample Requirement: Only 5 nanograms of sample per grid are needed. That’s a very tiny amount—much less than earlier methods.
- Faster Imaging: The magnetic beads are easy to see, so scientists can quickly find areas with useful particles in the microscope.
- Smart Software – DuSTER (Duplicated Selection to Exclude Rubbish):
- It helps remove bad or blurry images and keep only the clear ones.
- It picks each particle twice and only keeps it if the location matches both times.
- MagIC works with samples that are 100 times more dilute than what cryo-EM could handle before.
[UPSC 2023] ‘Aerial metagenomics’ best refers to which one of the following situations?
Options: (a) Collecting DNA samples from air in a habitat at one go* (b) Understanding the genetic makeup of avian species of a habitat (c) Using air-borne devices to colect blood samples from moving animals (d) Sending drones to inaccessible areas to collect plant and animal samples from land surfaces and water bodies |
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