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Subject: Biotech and Medical Sciences

  • Honey Adulteration in India

    10 out of 13 popular honey brands failed a key test of purity, the Centre for Science and Environment (CSE) has claimed in an investigation.

    Try this PYQ:

    Q.Consider the following kinds of organisms:

    1. Bat
    2. Bee
    3. Bird

    Which of the above is/are pollinating agent/agents?

    (a) 1 and 2 only

    (b) 2 only

    (c) 1 and 3 only

    (d) 1, 2 and 3

    Adulteration in honey

    • The CSE has resorted to the Nuclear Magnetic Resonance (NMR) testing to ascertain the composition of a product at the molecular level.
    • The NMR test is not required by Indian law for honey that is being marketed locally but is needed for export.
    • Current regulations specify around 18 parameters that honey must comply with for producers to label it ‘pure honey.
    • Among the tests employed as per Indian regulations is one to check whether the honey is adulterated with C4 sugar (cane sugar) or C3 sugar (rice sugar).
    • Most samples cleared these tests but failed another test called the Trace Marker for Rice test, to test for rice syrup adulteration.

    Significance of the CSE study

    • Adulteration of honey is a global problem with several countries, including India, devising regulations and new tests to check it.
    • It also destroys the livelihoods of bee-keepers who found it unprofitable to make pure honey because sugar-syrup honey was often available at half the price.
    • Some Indian companies in the honey business were importing synthetic sugar syrups from China to adulterate honey.
    • This shows how the business of adulteration has evolved so that it can pass the stipulated tests in India.

    Back2Basics: Nuclear Magnetic Resonance (NMR)

    • NMR spectroscopy is a crucial analytical tool for organic chemists.
    • It is a physical phenomenon in which nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and respond by producing an electromagnetic signal with a frequency characteristic of the magnetic field at the nucleus.
    • It is widely used to determine the structure of organic molecules in solution and study molecular physics and crystals as well as non-crystalline materials.
    • It is also routinely used in advanced medical imaging techniques, such as in magnetic resonance imaging (MRI).

  • Community Cord Blood Banking

    Community Cord Blood Banking, a stem cell banking initiative, has recently helped save the life of a girl child making it India’s first dual cord blood transplant through an unrelated donor.

    Must read:

    What is Cord Blood Banking?

    • Community Banking is a new sharing economy model of stem cell banking that was pioneered by LifeCell in India.
    • Parents who choose to store their child’s cord blood in a community bank will have access, in the event of medical need, to all of the other cord blood units in the bank.
    • A community bank is like a public cord blood bank in that the members are supporting each other, but it is also like a private bank because the members pay for this service and outsiders cannot participate.
    • It can fill an unmet health need in a country like India, where there is no national network of public banks and the population has unique genetics that are not covered by banks elsewhere in the world.
    • It is different from “hybrid” banking where both public and family banks share a laboratory, because in hybrid banks the pubic and family sides operate separately.
    • In a community bank the public and family functions are blended.

    Benefits of cord blood

    • It gives protection to a baby against all conditions treatable using stem cells (own & donor).
    • It gives protection to the baby’s siblings, parents and grandparents (maternal & paternal) by providing unrelated donor stem cells.

    Back2Basics: Stem Cell Therapy

    • It is a type of treatment option that uses a patient’s own stem cells to repair damaged tissue and repair injuries.
    • It is used to treat more than 80 disorders including neuromuscular and degenerative disorders. Eg. Bone-marrow transplant is used in Leukemia (blood cancer), sickle-cell anemia, immunodeficiency disorders.
    • Stem cells are usually taken from one of the two areas in the patient’s body: bone marrow or adipose (fat) tissue in their upper thigh/abdomen.
    • Because it is common to remove stem cells from areas of stored body fat, some refer to stem cell therapy as “Adipose Stem Cell Therapy” in some cases.

  • What is mRNA vaccine?

    The Moderna and Pfizer vaccines which recently announced their success use the same technology, based on messenger RNA, or mRNA.

    Try this PYQ first:

    Q.‘RNA interference (RNAi)’ technology has gained popularity in the last few years. Why?

    1. It is used in developing gene silencing therapies.
    2. It can be used in developing therapies for the treatment of cancer.
    3. It can be used to develop hormone replacement therapies.
    4. It can be used to produce crop plants that are resistant to viral pathogens.

    Select the correct answer using the code given below:

    (a) 1, 2 and 4

    (b) 2 and 3

    (c) 1 and 3

    (d) 1 and 4 only

    What is mRNA?

    • Messenger RNA (mRNA) is a single-stranded RNA (Ribo Nucleic Acid) molecule that is complementary to one of the DNA strands of a gene.
    • The mRNA is an RNA version of the gene that leaves the cell nucleus and moves to the cytoplasm where proteins are made.
    • During protein synthesis, an organelle called a ribosome moves along the mRNA, reads its base sequence, and uses the genetic code to translate each three-base triplet, or codon, into its corresponding amino acid.

    What is the mRNA vaccine?

    • Such vaccines make use of the messenger RNA molecules that tell the body’s cells what proteins to build.
    • The mRNA, in this case, is coded to tell the cells to recreate the spike protein of the coronavirus SARS-CoV-2, which causes Covid-19.
    • It is the spike protein — which appears as spikes on the surface of the coronavirus — that initiates the process of infection; it allows the virus to penetrate cells, after which it goes on to replicate.
    • A coronavirus vaccine based on mRNA, once injected into the body, will instruct the body’s cells to create copies of the spike protein.
    • In turn, this is expected to prompt the immune cells to create antibodies to fight it.
    • These antibodies will remain in the blood and fight the real virus if and when it infects the human body.

    Back2Basics: Ribo Nucleic Acid (RNA)

    • RNA is an important biological macromolecule that is present in all biological cells.
    • It is principally involved in the synthesis of proteins, carrying the messenger instructions from DNA, which itself contains the genetic instructions required for the development and maintenance of life.
    • In some viruses, RNA, rather than DNA, carries genetic information.
    • The type of RNA dictates the function that this molecule will have within the cell.
    • Aside from the coding region of messenger RNA (mRNA) molecules that will be translated into proteins, other cellular RNA elements are involved in different processes.

  • Kawasaki Disease

    Children in the world over have shown to be affected by either Kawasaki Disease (KD) since the reopening of schools.

    Try this PYQ:

    Q.H1N1 virus is sometimes mentioned in the news with reference to which one of the following diseases?

    (a) AIDS

    (b) Bird flu

    (c) Dengue

    (d) Swine flu

    What is Kawasaki Disease?

    • Kawasaki disease is an illness that causes blood vessels to become inflamed, almost always in young children.
    • Its cause is yet unknown. It is one of the leading causes of heart disease in kids.
    • But doctors can treat it if they find it early. Most children recover without any problems.

    Symptoms

    Kawasaki disease comes on fast, and symptoms show up in phases. Signs of the first phase of Kawasaki disease include:

    • High fever that lasts more than 5 days
    • Swelling and redness in hands and bottoms of feet
    • Red eyes
    • Swollen glands, especially in the neck
    • Irritated throat, mouth, and lips

    In the second phase, symptoms include:

    • Joint pain
    • Stomach trouble, such as diarrhoea and vomiting
    • Peeling skin on hands and feet

  • What is the D614G mutation in coronavirus?

    While novel coronavirus is undergoing many mutations, one particular mutation called D614G, according to a study, has become the dominant variant in the global COVID-19 pandemic.

    Try this PYQ:

    Q.Which one of the following statements is not correct?

    (a) Hepatitis B virus is transmitted much like HIV.

    (b) Hepatitis B, unlike Hepatitis C, does not have a vaccine.

    (c) Globally, the number of people infected with Hepatitis B and C viruses is several times more than those infected with HIV.

    (d) Some of those infected with Hepatitis B and C viruses do not show the symptoms for many years.

    D614G mutation

    • When the virus enters an individual’s body, it aims at creating copies of itself. When it makes an error in this copying process, we get a mutation.
    • In this case, the virus replaced the aspartic acid (D) in the 614th position of the amino acid with glycine (G). Hence the mutation is called the D614G.
    • This mutated form of the virus was first identified in China and then in Europe. Later it spread to other countries like the U.S. and Canada and was eventually reported in India.

    Threats posed

    • This particular mutation aids the virus in attaching more efficiently with the ACE2 receptor in the human host, thereby making it more successful in entering a human body than its predecessors.
    • D614G show increased infectivity but it also displayed greater ability at attaching itself to the cell walls inside an individual’s nose and throat, increasing the viral load.

    How prevalent is it in India?

    • A study (reveals that the D614G was one of the most prevalent spike mutations even during the initial phase of the pandemic.
    • Since then, D614G mutation’s ‘relative abundance’ has increased over time to 70% and above, in most states except Delhi.

  • Anomaly over Normal Body Temperature

    For several years now, doctors and researchers have known that 98.6°F is not really the gold-standard “normal” body temperature it was once considered to be.

    The “normal” body temperature

    • In 1851, Carl Reinhold August Wunderlich pioneered the use of the clinical thermometer.
    • It was a rod a foot long, which he would stick under the armpits of patients at the hospital attached with Leipzig University, and then wait for 15 minutes for the temperature to register.
    • He took over a million measurements of 25,000 patients, and published his findings in a book in 1868, in which he concluded that the average human body temperature is 98.6°F.
    • Most modern scientists feel Wunderlich’s experiments were flawed, and his equipment inaccurate.
    • Another study concluded that the average human body temperature is closer to 98.2°F, and suggested that the 98.6°F benchmark be discarded.

    The anomaly

    • Studies in the US and Europe have found average body temperatures declining over time.
    • In recent years, however, different studies have found the human body temperature averaging out differently, including at 97.7°, 97.9° and 98.2°F.
    • One of the largest such studies, published last year, found that body temperatures among Americans have been declining over the last two centuries.

    Now try this PYQ based on health sciences

    Q.Which of the following diseases can be transmitted from one person to another through tattooing?

    1. Chikungunya
    2. Hepatitis B
    3. HIV-AIDS

    Select the correct answer using the codes given below:

    (a) Only 1

    (b) 2 and 3 only

    (c) 1 and 3 only

    (d) 1, 2 and 3

  • Debate over Plasma Therapy’s efficacy

    Recently published findings on convalescent plasma therapy on Covid-19 patients have triggered a debate over its efficacy.

    Plasma Therapy

    • Plasma is the liquid part of the blood. Convalescent plasma, extracted from the blood of patients recovering from an infection, is a source of antibodies against the infection.
    • The therapy involves using their plasma to help others recover. For Covid-19, this has been one of the treatment options.
    • The donor would have to be a documented case of Covid-19 and healthy for 28 days since the last symptoms.

    How is it done?

    • The process to infuse plasma in a patient can be completed quickly.
    • It only requires standard blood collection practices, and extraction of plasma.
    • If whole blood is donated (350-450 ml), a blood fractionation process is used to separate the plasma.
    • Otherwise, a special machine called aphaeresis machine can be used to extract the plasma directly from the donor.
    • While blood is indeed extracted from the donor, the aphaeresis machine separates and extracts the plasma using a plasma kit, and the remaining blood components are returned into the donor’s body.

    WHO’s guidelines

    • WHO guidelines in 2014 mandate a donor’s permission before extracting plasma.
    • Plasma from only recovered patients must be taken, and donation must be done from people not infected with HIV, hepatitis, syphilis, or any infectious disease.
    • If whole blood is collected, the plasma is separated by sedimentation or centrifugation, then injected in the patient.
    • If plasma needs to be collected again from the same person, it must be done after 12 weeks of the first donation for males and 16 weeks for females, the WHO guidelines state.

    What has happened to spark the debate?

    • An ICMR study has found convalescent plasma was not associated with a reduction in progression to severe Covid-19 or all-cause mortality.
    • While the use of this therapy seemed to improve the resolution of shortness of breath and fatigue in patients with moderate Covid-19, this did not translate into a reduction in 28-day mortality or progression to severe disease.
    • Progression to severe disease or death at 28 days after enrolment occurred in 44 (19%) of the participants in the intervention arm as compared to 41 (18%) in the control arm.

    What happens if ICMR does remove the therapy from its guidelines?

    • The authorisation of convalescent plasma as a treatment for Covid-19 in India has led to questionable practices such as calls for donors on social media, and the sale of convalescent plasma on the black market.
    • The ICMR has been cautious because of the trial findings.
    • However, those guidelines are not necessarily binding and it is too early to dismiss convalescent plasma therapy. But there are other issues.
    • The therapy involves resource-intensive processes such as plasmapheresis, plasma storage, and measurement of neutralizing antibodies.

    Way ahead

    • This is a new virus, and around the world, the evidence is still emerging on the best therapeutic options.
    • Covid care is individualized care. Use of the right medication on the right patient does work.
    • Some of the therapies can be continued on compassionate grounds.
    • However, the potential harms of the non-immune components of convalescent plasma should be rigorously investigated.
    • Only donor plasma with detectable titers of neutralizing antibodies should be given to trial participants, to ensure that the potential for benefit exists for all intervention arm patients.

    Try this question:

    Q.What is convalescent plasma therapy? Discuss its efficacy and limitations for COVID-19 treatment.

  • Discovering the Tubarial Glands

    Researchers from the Netherlands have discovered a new location of salivary glands.

    Try this PYQ:

    Q.With references to the scientific progress of ancient India, which of the statements given below are correct?

    1. Different kinds of specialized surgical instruments were in common use by 1st century AD.
    2. Transplant of internal organs in the human body had begun by the beginning of 3rd century AD.
    3. The concept of sine of an angle was known in 5th century AD.
    4. The concept of cyclic quadrilaterals was known in 7th century AD.

    Select the correct answer using the code given below:

    (a) 1 and 2 only

    (b) 3 and 4 only

    (c) 1, 3 and 4 only

    (d) 1, 2, 3 and 4

    Tubarial Glands

    • The salivary gland system in the human body has three paired major glands and over 1,000 minor glands that are spread throughout the mucosa.
    • These glands produce saliva necessary for swallowing, digestion, tasting, mastication and dental hygiene.
    • When researchers were studying scans from about 100 people, they found a bilateral structure at the back of the nasopharynx and these glands had characteristics of salivary glands.
    • Researchers have proposed the name “tubarial glands” for their discovery.
    • The researchers believe that these glands would qualify as the fourth pair of major salivary glands.
    • The proposed name is based on their anatomical location; the other three glands are called parotid, submandibular and sublingual.

    Why are these glands being discovered only now?

    • The location of these glands is at a poorly accessible anatomical location under the base of the skull, which is an area that can only be visualized using nasal endoscopy.
    • Further, conventional imaging techniques such as a CT scan, MRI and ultrasound have not allowed the visualization of these glands.
    • For the scans done on the 100 patients, a new type of scan called the PSMA PET/CT scan was used, which was able to provide the high sensitivity and specificity required to detect these glands.

    What is the purpose of these glands?

    • So far, researchers suspect that the physiological function of the glands is to moisten and lubricate the nasopharynx and the oropharynx.
    • However, this interpretation needs to be confirmed with additional research.

    Significance of this discovery

    • The discovery is potentially good news for some cancer patients with head and neck cancers.
    • Patients with head and neck cancers and tumours in the tongue or the throat are treated with radiation therapy that can damage the new salivary glands, whose location was not previously known.
    • Oncologists will be able to circumvent these areas and protect them from the side effects of radiation which can lead to complications such as trouble speaking, swallowing and speaking.
    • Some patients may even face an increased risk of caries and oral infections that can significantly impact their life.

  • Need for guidelines for gene-editing research in India

    The Nobel Prize in Chemistry for 2020 has been awarded for the discovery of CRISPR Cas9. The two scientists have pioneered the use of CRISPR  – Cas9 (CRISPR-associated protein 9) system as a gene-editing tool.

    Background of discovery of CRISPR

    • In 1987a group of Japanese researchers observed an unusual homologous DNA sequence bearing direct repeats with spacing in a eubacterial gene.
    • In subsequent years CRISPR was discovered and showed to be a bacterial adaptive immune system and to act on DNA targets.
    • A notable discovery on the use of CRISPR as a gene-editing tool was by a Lithuanian biochemist, Virginijus Šikšnys, in 2012.
    • Šikšnys showed that Cas9 could cut purified DNA in a test tube, the same discovery for which both Charpentier and Doudna were given the credit.
    • Thus, the exclusion of Siksnys from this year’s Nobel is going to raise discussions.

    Issue of gene-edited babies

    • The world was alarmed by such a mission in 2018 when Chinese scientist edited genes in human embryos using the CRISPR-Cas9 system which resulted in the birth of twin girls.
    • The incident became known as the case of the first gene-edited babies of the world.
    • Following the incident, the World Health Organization formed a panel of gene-editing experts.
    • The expert panel suggested a central registry of all human genome editing research in order to create an open and transparent database of ongoing work.

    Guidelines and regulations in India

    • In India, several rules, guidelines, and policies are notified under the Environment Protection Act, 1986 to regulate genetically modified organisms.
    • The above Act and the National Ethical Guidelines for Biomedical and Health Research involving human participants, 2017, by the Indian Council of Medical Research (ICMR), and the Biomedical and Health Research Regulation Bill implies regulation of the gene-editing process.
    • This is especially so in the usage of its language “modification, deletion or removal of parts of heritable material”.
    • However, there is no explicit mention of the term gene editing.

    Consider the question “What is CRISPR-Cas9? How it helps in the gene-editing? What are the concerns with use of it for gene-editing?”

    Conclusion

    It is time that India came up with a specific law to ban germline editing and put out guidelines for conducting gene-editing research giving rise to modified organisms.

    Back2Basics: What is CRISPR?

    • CRISPRs: “CRISPR” stands for “clusters of regularly interspaced short palindromic repeats.”
    • It is a specialized region of DNA with two distinct characteristics: the presence of nucleotide repeats and spacers.
    • Repeated sequences of nucleotides — the building blocks of DNA — are distributed throughout a CRISPR region.
    • Spacers are bits of DNA that are interspersed among these repeated sequences.
    • In the case of bacteria, the spacers are taken from viruses that previously attacked the organism.
    • They serve as a bank of memories, which enables bacteria to recognize the viruses and fight off future attacks.

  • 2020 Nobel for Hepatitis C Virus discovery

    Americans Harvey J Alter and Charles M Rice, and British scientist Michael Houghton were awarded the Nobel Prize for Medicine or Physiology on Monday for the discovery of the hepatitis C virus.

    Try this PYQ:

    Q.Which one of the following statements is not correct? (CSP 2019)

    (a) Hepatitis B virus is transmitted much like HIV.

    (b) Hepatitis B. unlike Hepatitis C, does not have a vaccine.

    (c) Globally, the number of people infected with Hepatitis B and C viruses arc several times more than those infected with HIV.

    (d) Some of those infected with Hepatitis B and C viruses do not show the symptoms for many years.

    Hepatitis C Virus

    • Hepatitis C virus (HCV) is a bloodborne virus and causes liver diseases. It refers to an inflammatory condition of the liver.
    • The novel virus caused several deaths in the 1960s and 1970s — but remained unknown until its discovery in the late 1980s.

    What are other Hepatitis Viruses?

    • Before the discovery of the Hepatitis C virus, two other viruses were known to cause hepatitis in patients.
    • The Hepatitis A virus was known to spread mainly through contaminated food and water and caused a relatively milder form of liver inflammation.
    • Hepatitis B, discovered in the 1960s, was known to transmit mainly through infected blood and caused a more serious form of the disease.
    • Incidentally, the discovery of the Hepatitis B virus too was rewarded with a Nobel Prize in Medicine, given to Baruch Blumberg in 1976. There are vaccines available for this disease now.

    How Hepatitis C came to observation?

    • The discovery and identification of the Hepatitis B virus facilitated the development of a diagnostic test to detect its presence in blood.
    • Thereafter, only blood sanitized from this virus would be given to patients, but it was observed that even this sanitized blood was able to prevent only 20% of the blood-borne hepatitis cases.
    • It was then that the search for the new virus began.

    How is Hepatitis C treated?

    • Presently there is no vaccine available for HCV. However, it can be treated with antiviral medication.
    • Hepatitis A and B are preventable by vaccine.

    Back2Basics:

    https://www.civilsdaily.com/news/in-news-nobel-prize/